Antisense oligonucleotides are synthetic oligomers that bind a target mRNA or DNA, forming duplexes and thereby blocking its translation into a peptide or triggering its degradation.
Among several DNA analogs proposed as antisense DNA drugs, antisense DNA in which the backbone is modified into phosphorothioate is the most promising for therapeutic application because of its resistance to nuclease degradation.
This methodology is a useful tool for synthesis control and analysis of phosphorothioate analogs and for the determination of their subcellular distribution, in vitro stability, tissue half-life, purity, and metabolism.
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