In temperature gradient CE (TGCE), a continuous, gradually decreasing temperature gradient is established during the electrophoresis. This approach can be applied to detection of DNA mutations based on thermodynamic stability and mobility shift during electrophoresis.
By spanning a wide temperature range, it is possible to perform simultaneous heteroduplex analysis for various mutation types that have different melting temperatures. Genomic DNA also can be amplified with one fluorescence-labeled primer and one GC-clamped primer and analyzed by TGCE under denaturing temperature conditions.
This tactic has been applied to the detection of mutations in exon 8 of the p53 gene from tumor samples and controls.
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